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Pioneer Data Systems Inc

Scientist (Cell Lines / PCR / Elisa)

Post Date:Jan 17, 2018Type:Full-Time
Start Date:12/29/2017Salary:
Location:US-PA-Spring House Job Reference:SC-SNTS-W523
Education, Experience: 
  • M.S. in Biomedical Engineering, Molecular Biology, Cell biology, Biochemistry, Chemistry or a related field with 2+ years industry experience. 
  • Supervision required: Minimal

Job Description:
Position Details:
  • Job Title: Scientist (Cell Lines / PCR / Elisa)
  • Duration: 23 months contract
  • Location: Spring House, PA
  • Client has the right-to-hire you as a permanent employee at any time during or after the end of contract.
  • You may participate in the company group medical insurance plan which includes dental and vision.
Job Description:
  • The candidate will contribute to the evaluation and development of scalable AAV manufacturing technologies in support of efforts to develop human therapies. She/he will work to engineer a mammalian expression host as well as to help evaluate alternative expression systems such as baculovirus. The scientist will work to develop cell lines that will be used in GMP manufacturing of AAV-based therapies. 
Job Responsibilities:
  • The candidate will work to engineer a mammalian expression host for large-scale AAV production. This entails development of stable mammalian cell lines including transfection, selection, clone screening and cell banking. Screening will include assessment of transgene stability and AAV production upon scale-up and induction. 
  • Strong molecular biology and cell culture experience is essential. Small scale AAV production and characterization in mammalian cell culture or Baculovirus systems for the assessment of manufacturing systems and/ or to support target validation activities is highly desirable. 
  • Experience developing and characterizing stable mammalian cell lines is required. The candidate should have experience performing routine quantitative assays including qPCR, RT-PCR, Western Blot, ELISA and others. 
  • The ability to generate and maintain experimental data in an electronic laboratory notebook in a highly organized manner is essential. 
  • Uses the principles and theories of science and mathematics to solve problems in R&D and to help invent and improve products-processes. 
  • Performs qualitative and quantitative chemical analysis of raw materials. 
  • Coordinates testing responsibilities to ensure QA test support according to the needs of Manufacturing to maintain continuous process flow. 
  • Functions as a team member in the QA Chemical Laboratories to provide timely test analysis information on the status of conformance to requirements. 
  • Investigates testing abnormalities and implements corrective action. Investigates testing abnormalities and implements corrective action. 
  • Assists with determination of root causes of non-conformances and recommends corrective actions. Audits documentation, device history records, and test methods for compliance. 
  • Assists with special projects by supporting the development of new test methods of performing investigative test analyses as required. 
  • Assists in projects including the validation of test methods and transfer of new products-processes-test methods.

Contact Information
Pioneer Data Systems Inc
Name: Sarah Chawhan
Ph: 732-947-3451

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